megatomi.com for Dummies
megatomi.com for Dummies
Blog Article
Our new Obvious+ tissue clearing system is the only strategy that delipidates samples without improve in morphology and with minimal impact on structural integrity.
Megatome is actually a vibrating microtome intended to portion a broad selection of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With large blade vibrating frequency and minimized blade deflection, Megatome allows significant-throughput tissue sectioning with uniform area profile, and minimal tissue harm and information loss.
Antibodies might consider weeks to diffuse via only a few millimeters of tissue, having a steep labeling gradient from surface to core.
Megatome is designed for precision: the blade vibrates at a higher frequency and larger amplitude assortment than other microtomes, and incorporates a special deflection Handle mechanism.
SE employs a rotational electrical area to disperse extremely electromobile molecules (like antibodies or surfactant micelles) all over a porous sample with out damaging electrically charged structures throughout the tissue. This allows two-4 day clearing of intact organs,
job to produce an aggregated Javadoc. Within this occasion, javadocTask really should be set to allJavadoc to make sure that the right documentation is developed prior to creating the docset.
Megatome is a novel microtome that enables for prime-precision sectioning of a wide range of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with negligible tissue injury and information reduction.
eFLASH is really a immediate tissue labeling strategy that allows for uniform full-organ staining in twenty rounds of labeling.
The SmartSPIM light sheet microscope supplies unparalleled resolution and acquisition speed through intact tissue volumes, whilst its modular style and design enables you to up grade and customize particular person elements.
Docset development requires at megatomi.com bare minimum two options: the identify from the docset and The placement of the Javadoc documents to incorporate inside the docset.
This will produce a docset named Sample in The existing Listing. Docset generation may be personalized with optional arguments:
Either obtain a launch or make a distribution zip as outlined over. Unzip the archive into a wished-for place.
--displayName: Will set the identify as revealed in Dash. This can be helpful for those who create a docset with title SampleProject but Exhibit identify Sample Venture as an alternative. This placing will default to the value of --title if omitted.
Our novel SHIELD tissue preservation method kinds intramolecular bonds working with polyfunctional, adaptable epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.